Josh Phegley had trouble hearing the first question of his postgame media scrum late Friday night, the result of some sort of ear problem that’s been bugging him.Phegley isn’t alone in struggling to comprehend whatever the heck it was that transpired at PNC Park, as the Athletics crushed the Pirates, 14-1, and Phegley had a game for the ages.Oakland’s No. 8 hitter had seven RBIs by the fourth inning and finished with eight after he smacked a solo home run in the ninth inning. The 14 runs the …
The fact that protein machines use energy to undergo conformational rearrangements, and that these “moving parts” perform functional work, places them squarely in the realm of machinery – except on a scale so tiny, their operations are only now coming to light.1. Valeria V�squez and Eduardo Perozo, “Structural Biology: A channel with a twist,” Nature 461, 47-49 (3 September 2009) | doi:10.1038/461047a.2. Liu, Gandhi and Rees, “Structure of a tetrameric MscL in an expanded intermediate state,” Nature 461, 120-124 (3 September 2009) | doi:10.1038/nature08277.3. Cook, Fukuhara, Jinek and Conti, “Structures of the tRNA export factor in the nuclear and cytosolic states,” Nature 461, 60-65 (3 September 2009) | doi:10.1038/nature08394.4. Guydosh and Block, “Direct observation of the binding state of the kinesin head to the microtubule,” Nature 461, 125-128 (3 September 2009) | doi:10.1038/nature08259.Molecular machines – the very concept is only a couple of decades old. This is phenomenal. It is marvelous and wonderful beyond description. You can almost sense the astonishment and excitement of these biophysicists uncovering these tiny wonders in the cell. Who could have imagined this is how life works? Think of the centuries, the millennia, of people going about their business, oblivious to the fact that at scales too tiny to imagine a whole factory of automated molecular machines was keeping them alive. The few thinkers after the discovery of cells by Robert Hooke envisioned little people (homunculi) doing some of it, but our instruments were too coarse to elucidate the workings inside till recently – till our generation. Next to the discovery of DNA and the genetic code this must be considered one of the most important discoveries in the history of science. If Antony van Leeuwenhoek was astonished at what he saw with his primitive hand lens, how much more should we be flabbergasted at what is coming into focus, now that we can discern the activity of individual molecules? The Darwinists are strangely silent about all this. In our 9 years of reporting, very few papers on molecular machines have even mentioned evolution (e.g., 10/02/2001, 01/09/2002), and those that did usually just assumed it rather than tried to seriously explain how the most primitive life-forms could have became endowed with factories of mechanical filters, scribes, taxicabs and walking robots by chance (e.g., 09/16/2000, 08/24/2009 08/26/2005). Search on “molecular machines” in the search bar above and check. There are lots of examples. It’s time to cast off that antiquated 19th-century mindset that tried to imagine all this from the bottom up. Let us regard as silly the tales of miracles of “emergence” occurring mindlessly in “a chance Motion of I don’t know what little Particles,” as Christiaan Huygens, our Scientist of the Month, quipped. Paley is back with a vengeance. The contrivances of nature are more wonderful than he or any other philosopher or scientist could have imagined. It’s a Designed world after all. Rejoice, give thanks and sing!(Visited 10 times, 1 visits today)FacebookTwitterPinterestSave分享0 Scientific papers continue to exhibit the exquisite mechanisms in the cell for handling all kinds of situations, through the operation of molecular machines. Here are a few recent examples from this week’s issue of Nature (Sept 3, 2009).Molecular sieve: What happens when a cell gets bloated? Too much water entering a cell can increase the pressure against the membrane, “potentially compromising the integrity of the cell,” said Valeria V�squez and Eduardo Perozo in Nature this week.1 They described findings about a molecular sieve named MscL by Liu et al in the same issue of Nature.2 MscL in bacteria is made up of multiple protein parts that form a pore in the cell membrane. The research team from Caltech and Howard Hughes Medical Institute found that the components flatten out and pivot, opening up the pore like an iris when sufficient pressure is applied. This is called “mechanosensation” because it operates automatically via mechanical pressure. “These channels act as ‘emergency relief valves,’ protecting bacteria from lysis [disruption] upon acute osmotic down-shock,” the authors said. “MscL has a complex gating behaviour; it exhibits several intermediates between the closed and open states, including one putative non-conductive expanded state and at least three sub-conducting states.” The team’s contribution was to image one of the intermediate states. The research paper did not mention evolution. V�squez and Perozo, however, said, “free-living cells have evolved a variety of mechanisms to deal with sudden variations in the physicochemical properties of their surroundings,” and later said, “Most prokaryotes (bacteria and archaea) have therefore evolved a ‘pressure-release valve’ mechanism in which changes in membrane tension open up channels to form large, aqueous pores in the membrane,” but they did not explain how evolution could have accomplished this. They made it sound like the bacteria purposely employed evolution (whatever they meant by the term) to solve a real problem. They did not explain how bacteria got through osmotic down-shock without the pressure release valves.Molecular taxicab: Transfer RNAs (tRNA) are made in the nucleus but need to commute to work outside, in the cytoplasm, where the ribosomes are. They are small enough to barely squeeze through the nuclear pore complex (NPC) – the complicated gates in the nuclear membrane that control traffic in and out – but they don’t avail themselves of that freedom, lest their exposed parts interact with the authentication mechanisms of the NPC. Instead, they hale a taxicab to escort them through. That taxicab, or “tRNA export factor,” is called Xpot. Xpot is a complex molecule that fits around the exposed parts of the tRNA. It literally “wraps around” the tRNA, undergoing conformational changes as it clamps on. Imagine a taxicab wrapping around you, and you get the picture. Xpot is general enough to fit all 20 kinds of tRNAs, but specific enough to protect their delicate active sites. It is also able to recognize and reject tRNAs that are immature. Only tRNAs that have passed a processing exam are allowed in the taxi. The authors of a paper in Nature who studied Xpot said, “Xpot undergoes a large conformational change on binding cargo, wrapping around the tRNA and, in particular, binding to the tRNA 5′ and 3′ ends. The binding mode explains how Xpot can recognize all mature tRNAs in the cell and yet distinguish them from those that have not been properly processed, thus coupling tRNA export to quality control.”3 As an additional control, Xpot does not interact with tRNA except in the presence of another factor in the nucleus called RanGTP. After safe transport through the nuclear pore complex, another factor in the cytoplasm unlocks the RanGTP, allowing the Xpot taxicab to unwrap from the tRNA. The tRNA then heads off to the ribosome to fulfill its work shift as a scribe, translating the genetic code into the protein code. “Transfer RNAs are among the most ubiquitous molecules in cells,” they said, “central to decoding information from messenger RNAs on translating ribosomes.” The authors of the paper did not discuss how Xpot originated, but six times they said that parts of Xpot are either “conserved,” “evolutionarily conserved” or “highly conserved” (i.e., unevolved) throughout the living world.Molecular sherpa: Kinesin is among the most fascinating molecular machines in the cell, because it literally “walks” hand-over-hand on microtubule trails, carrying cargo. In doing this, it converts chemical energy from ATP into mechanical work. Writing in this week’s Nature,4 Guydosh and Block of Stanford described direct observation of the binding state of the hands (called heads) of kinesin to the microtubule. They found that it walks tiptoe on the tightrope: “Here we report the development of a single-molecule assay that can directly report head binding in a walking kinesin molecule, and show that only a single head is bound to the microtubule between steps at low ATP concentrations.” The rear head has to unbind before the forward head can bind. This keeps the kinesin from getting stuck with both feet (heads) on the tightrope. If you can stand some jargon, here is what they said about the complexities of how this works:The inability of one head to bind the microtubule offers a natural explanation for the observation that the microtubule-stimulated release of ADP is inhibited until the microtubule-attached head binds ATP and docks its neck linker (Fig. 4, state 2). Strain produced by an unfavourable neck-linker conformation also explains the observation that ATP does not bind prematurely to the front, nucleotide-free head of a 2-HB kinesin molecule (Fig. 4, state 3). Any tight binding of ATP is disfavoured because it is coupled to neck-linker docking and, therefore, to the generation of a strained configuration in which both neck linkers are docked (Fig. 4, S3). We anticipate that the single-molecule techniques presented here will be applicable to the study of dynamic properties of other motors and macromolecules that undergo analogous conformational rearrangements.
26 June 2006(FNB) and the Gauteng Department of Housing are co-operating to close the gap that exists in South Africa’s affordable housing market.FNB, one of South Africa’s “big four” retail banks, launched a R800-million housing development last Thursday that will see more than 3 000 houses being built in a new Soweto suburb to be called Glen Ridge.Each housing unit will consist of three bedrooms, a bathroom, kitchen and lounge at the cost of R168 000 (all inclusive).Construction will begin in July, with the first batch of houses to be available from as early as October.The project is split into commercial property finance (to fund developers to build the houses) and end-user finance in home loans for potential buyers to address the challenge of access to credit.FNB has set aside approximately R500-million to provide finance for the buyers, and R300-million for established property developers to build the houses.The initiative is in line with an agreement the government signed last year with the country’s four major banks – FNB, Absa, Standard Bank and Nedbank – under which the institutions committed themselves to pouring R42-billion into the country’s low-cost housing market.Gauteng Housing MEC Nomvula Mokonyane commended the bank for helping ordinary South Africans to acquire homes through innovative home loan products.“Our role as provincial government will be to provide housing subsidies as equity to ensure affordability and quality houses for people in the middle to low income bracket,” Mokonyane said.Township development has in the past had limited appeal for developers because of difficulties involved in selling the properties.However, FNB chief executive officer Michael Jordaan said the collaboration between the banks and the government would make the provision of finance to buyers far smoother and the initiative an attractive one for developers.Last month, FNB unveiled a R368-million project to build more than 1 000 affordable houses at Cosmo City, north of Johannesburg. So far this year, the bank has ploughed approximately R1.7-billion into affordable housing developments.Jordaan emphasised that supporting South Africa’s previously under-serviced housing market was not merely about “ticking FSC [Financial Sector Charter] target boxes.”It was a business imperative, he said, for the country’s banks to contribute to the sustainable development and economic well-being of the communities they served.Source: BuaNews Want to use this article in your publication or on your website?See: Using SAinfo material
The Beermen won a fifth straight all-Filipino crown, surviving a crucial error and missed free throws in the stretch to hang on to a 72-71 victory over a stubborn Magnolia crew Wednesday night at Smart Araneta Coliseum.Alex Cabagnot nailed a jumper at the baseline for the lead in the last 57.2 seconds to ice the final count.FEATURED STORIESSPORTSPrivate companies step in to help SEA Games hostingSPORTSPalace wants Cayetano’s PHISGOC Foundation probed over corruption chargesSPORTSSingapore latest to raise issue on SEA Games food, logistics“It was extremely difficult,” Cabagnot said of the series, which swapped winners from Game 1 until San Miguel Beer won back-to-back in the final two games. “We just went back and forth.”“Credit goes to the players. They made me look like a good coach,” said San Miguel Beer coach Leo Austria in Filipino. “We just got lucky tonight.” Duterte wants probe of SEA Games mess Ethel Booba twits Mocha over 2 toilets in one cubicle at SEA Games venue “I just want to thank God that no one got hurt even if it was a physical series,” said Fajardo, who averaged 22.9 points, 19.1 rebounds and 1.9 blocks a game in the Finals.Sports Related Videospowered by AdSparcRead Next View comments PH underwater hockey team aims to make waves in SEA Games PLAY LIST 02:42PH underwater hockey team aims to make waves in SEA Games01:44Philippines marks anniversary of massacre with calls for justice01:19Fire erupts in Barangay Tatalon in Quezon City01:07Trump talks impeachment while meeting NCAA athletes02:49World-class track facilities installed at NCC for SEA Games02:11Trump awards medals to Jon Voight, Alison Krauss LATEST STORIES Two-day strike in Bicol fails to cripple transport Don’t miss out on the latest news and information. DA eyes importing ‘galunggong’ anew He wasn’t kidding.Ian Sangalang missed a short jumper going into the final 40 seconds but the Beermen could not control the rebound—on a night, ironically, that June Mar Fajardo set the single-game record for rebounds with 31. Jio Jalalon missed a jumper in the next possession but Marcio Lassiter threw the ball away under pressure to give Magnolia 17 seconds to turn the game around.But Jalalon missed what looked like an easy short stab, and Fajardo collared the rebound with four seconds left.Magnolia put the reigning five-time MVP on the stripe and Fajardo missed both, but Paul Lee could not get past the backcourt as time ran out.As tense and unpredictable as the series was, the choice of Finals MVP was hardly a surprise.ADVERTISEMENT Catholic schools seek legislated pay hike, too MOST READ Charles Rhodes wants crown back ‘Rebel attack’ no cause for concern-PNP, AFP Cayetano: Senate, Drilon to be blamed for SEA Games mess Private companies step in to help SEA Games hosting Photo by Tristan Tamayo/INQUIRER.netIn a series that went the distance, it was only fitting that Game 7 would do the same thing.In the end, the San Miguel Beermen kept their dynastic grip on the PBA Philippine Cup—this latest title, however, they barely captured.ADVERTISEMENT
One person died and two others were seriously injured in a one-vehicle crash that occurred Saturday afternoon near Lesterville.The South Dakota’s Highway Patrol says the driver of a westbound Ford Mustang lost control of his vehicle on 430th Avenue.The vehicle went into ditch, hit a field approach, went airborne and caught fire after landing.All three occupants were ejected from the vehicle and airlifted to a Sioux Falls hospital.A 20-year-old passenger later died from his injuries.The 29-year-old driver and a 25-year-old second passenger both suffered life-threatening injuries.Charges are pending against the driver.The names of those involved have not been released and the accident remains under investigation.
About the authorPaul VegasShare the loveHave your say Wenger: Marseille coach AVB too young taking Chelsea jobby Paul Vegas17 days agoSend to a friendShare the loveFormer Arsenal boss Arsene Wenger is happy seeing Andre Villas-Boas in charge of Marseille.Wenger feels Villas-Boas took the Chelsea job too soon.He said, “He may have been a little young at the time when he came to Chelsea. He took little break and sometimes it does not hurt in mid-career. From what I have seen since the beginning of the season, his team has the desire. “It is a little limited in potential. Marseille today does not have the level to play the title of champion of France. But he managed to get the team behind him and instill a dynamic in the team. If he finishes in the first six, I think he has really had a good season.”
ANN ARBOR, MI – APRIL 04: Head coach Jim Harbaugh of the Michigan Wolverines listens in on an offensive huddle during the Michigan Football Spring Game on April 4, 2015 at Michigan Stadium in Ann Arbor, Michigan. (Photo by Gregory Shamus/Getty Images)Today is the 40th anniversary of the wreck of the SS Edmund Fitzgerald in Lake Superior off the coast of Michigan. The ship’s entire 29-man crew died in the tragedy. It’s a haunting historical event for many who live in the area. Michigan head coach Jim Harbaugh has already admitted that his favorite song is Gordon Lightfoot’s “The Wreck of the Edmund Fitzgerald” which is a tribute to the captain and crew of the ill-fated ship. This afternoon, Harbaugh tweeted out a link to Lightfoot’s song as a way of remembering those who perished in the storm 40 years ago. Remembering the captain and crew of the Edmund Fitzgerald today. God Bless You and Yours! https://t.co/OZl3bMYWuy— Coach Harbaugh (@CoachJim4UM) November 10, 2015Harbaugh truly is a Michigan Man, in more ways than one.
“This will help to prepare in the transition process to a single-shift system. It is important that we do so, because we are not doing so for ourselves, we are doing it for the future and for the transformation of St. James,” the Minister said. Minister of Education, Youth and Information, Senator the Hon. Ruel Reid, has reiterated that urgent steps are being taken by the Government to get schools across Jamaica, such as the St. James High School in Montego Bay, off the two-shift system.Speaking at a ceremony to mark the official opening of a new three-storey block of classrooms at the St. James High School on January 30, Mr. Reid said he is proud that despite the double-shift system, the institution has been performing extremely well.“I am in a hurry under my shift, to fix all of these problems. Yes, I have 42 schools still on shift and, systematically, we are going to take them off one by one,” the Minister said.Senator Reid noted that the Ministry responded quickly when St. James High requested financial support for additional classroom spaces and for the construction of a perimeter wall.“This will help to prepare in the transition process to a single-shift system. It is important that we do so, because we are not doing so for ourselves, we are doing it for the future and for the transformation of St. James,” the Minister said.Meanwhile, Senator Reid said the Ministry will be constructing five new high schools across the island, one of which will be in St. James, which will add significantly to the number of classroom spaces available in western Jamaica.“We have a lot of work to do, but, under God, we are going to get it done. I am getting full support, and I have the fiscal space to get it done,” he said.The Minister also visited Anchovy High School in St. James, and the Knockalva Agricultural School and the Knockalva Technical High School in neighbouring Hanover.Mr. Reid told JIS News that he was happy to have visited the institutions to get a first-hand look at their operations and make assessments and recommendations.“My tour has been extremely successful, and I am very happy to be able to get out of the office to see exactly what is happening in these institutions and to give some policy direction,” he added. Speaking at a ceremony to mark the official opening of a new three-storey block of classrooms at the St. James High School on January 30, Mr. Reid said he is proud that despite the double-shift system, the institution has been performing extremely well. Minister of Education, Youth and Information, Senator the Hon. Ruel Reid, has reiterated that urgent steps are being taken by the Government to get schools across Jamaica, such as the St. James High School in Montego Bay, off the two-shift system. Story Highlights
LEAVE A REPLY Cancel replyLog in to leave a comment Advertisement Twitter Residents on Coulter Court in Langley saw their neighbourhood transformed into a winter wonderland for five days. Not everyone got into the spirit. (Katya Stano) The Township of Langley touts itself as “one of the most film friendly municipalities in B.C.’s Lower Mainland,” but becoming a hit with the film industry has also produced a new drama: conflict between those who want film shoots and those who don’t.“One house makes all the money and the rest of us just have to put up with the noise and the set-up and no parking,” said Katya Stano who lives on Coulter Court.A five-day film shoot wrapped up Wednesday on Stano’s cul-de-sac. A location scout had gone door-to-door before production began and offered residents $250 if they agreed to have their properties covered in snow and Christmas decorations for the duration of the shoot. Advertisement Login/Register With: Advertisement Some neighbours felt the disruption was worth more and asked for $600. Stano says the owners of the house where the main filming occurred were rumoured to have received $1,000 a day.“It pits neighbours against each other,” she said, adding that the friction isn’t worth it. She declined the film company’s offer to put snow and decorations on her property for payment.As the number of film productions in B.C. rises, opposition has also grown. Proponents say these productions bring cash to municipalities. The film industry invested $35 million in the Township of Langley’s local economy last year. Facebook
Advertisement TORONTO, June 14, 2018 – A series of quirky videos and social media savvy helped Josée Caron and Lucy Nilesclinch the top spot in the 13th annual English SOCAN Songwriting Prize presented by YouTube Music. “Play The Field” was written by the Atlantic Canada natives and is performed by the band they co-front, Partner. The SOCAN Songwriting Prize is one of few competitions in Canada that award excellence in songwriting. Ten outstanding songs created by emerging songwriters over the past year are nominated by a panel of 15 esteemed music industry experts. The general public is then invited to vote daily for their favourite to determine the winner. SOCAN plays no role in determining the nominees or winners outside of ensuring they are members of SOCAN. A mirroring competition for songs in French, the Prix de la chanson SOCAN, is conducted separately.“Congratulations to Josée Caron and Lucy Niles on winning the 2018 SOCAN Songwriting Prize. In a competition that celebrates songwriting there was no shortage of great songs this year and winning was no easy feat,” said Michael McCarty, Chief Membership & Business Development Officer at SOCAN. “The diversity in genres, gender, and cultural influences truly showcased the breadth of not only our talent but the unique stories that Canadian songwriters have to tell. “Play The Field” is a force and we wish Josée and Lucy continued success in the early days of what is sure to be a long and successful music career.”Caron and Niles added, “‘Play the Field’ is one of our most personal songs, about an innocent time in a young person’s life. Writing it was an exciting experience. Josée made a funny demo and Lucy wrote her verse while working at Tim’s. We would like to thank all the music lovers and supporters for the huge opportunity and compliment. It is an honour to be nominated alongside so many talented songwriters.”The winner of the Prix de la chanson SOCAN is “56k” written by Simon Trudeau Cliche, Jeff Martinez, Marc Vincent; performed by LOUD and published by Productions Silence D’Or.The other nine songs nominated in the English category were:“Dreams Tonite” – written by Alec O’Hanley, Molly Rankin; performed by Alvvays; published by Rough Trade Publishing Canada.“Money” – written by Leandra Earl, Eliza Enman-McDaniel, Jordan Miller, Kylie Miller, Garrett Lee; performed by The Beaches; published by Done with Dolls Inc., Besme, administered by Kobalt Music Group Ltd.“Main Girl” – written by Charlotte Cardin; performed by Charlotte Cardin; published by Red Brick c/o Corico Arts.“Cotton Candy” – written by Jessie Reyez; performed by Jessie Reyez; published by BMG Rights Management Canada.“Chills” – written by James Barker, Gavin Slate, Travis Wood, Donovan Woods; performed by James Barker Band; published by Warner Chappell Music Canada, Ole Media Management LP II.“Walkaway” – written by Jasmyn Burke, Morgan Waters; performed by Weaves.“Magic”– written by Eoin Killeen, Timothy Law, Patrisha Sanna Campbell; performed by Birthday Boy and Trish.“Healers” – written by Benjamin McCarthy, Iskwé, Ryan Somerville; performed by Iskwé.“Lingua Franca” – written by Neil Bednis, Christopher Laurignano, Fraser McClean, Melanie St. Pierre; performed by Casper Skulls.The 2017 winner of the SOCAN Songwriting Prize was PUP for “DVP” written by band members Stefan Babcock, Nestor Chumak, Zachary Mykula, and Steven Sladkowski. Additional winners are available to view on the SOCAN Songwriting Prize website.About SOCANSOCAN connects more than four-million music creators worldwide and more than a quarter-million businesses and individuals in Canada. More than 150,000 songwriters, composers and music publishers are its direct members, and more than 100,000 organizations are Licensed To Play music across Canada. With a concerted use of progressive technology and unique data as well as a commitment to lead the global transformation of music rights, with wholly-owned companies Audiam and MediaNet, SOCAN is dedicated to upholding the fundamental truths that music has value and music creators and publishers deserve fair compensation for their work. For more information: www.socan.com Advertisement Twitter Login/Register With: LEAVE A REPLY Cancel replyLog in to leave a comment Facebook Advertisement Variety and balance were evident in the 2018 competition in which women dominated, several genres were represented and, for the first time, a country song landed in the top 10. Music fans made their voices heard as they voted for their favourite songs among the finalists, and in the end, Partner would prevail receiving the $10,000 cash prize, a Yamaha PSR-S970 Keyboard, and a $500 gift card from Long & McQuade.“We are so thrilled and honoured to be the recipients of the SOCAN Songwriting Prize,” said Caron and Niles. “Songwriting is one of our all-time greatest joys, and to be recognized by fellow music lovers in this capacity is a dream come true.”